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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Comp Neurol. 2013 Oct 1;521(14):10.1002/cne.23351. doi: 10.1002/cne.23351

Table 2.

of Primary Antibodies used

Antigen Immunogen (what the antibody was raised against; full sequence and species) Manufacturer, species antibody was raised in, mono- vs. polyclonal, catalog or lot number Dilution used
TuJ1 This antibody was raised against microtubules derived from rat brain. It is well characterized and highly reactive to neuron specific Class III β-tubulin (βIII) Covance Research Products Inc (San Diego), Purified mouse IgG2a monoclonal Antibody, Unconjugated, Clone TUJ1, #MMS-435P-100 1:500
HNK1 E10 chick optic nerve Developmental Studies Hybridoma Bank, (Univ. Iowa). Mouse monoclonal VC1.1 hybridoma. 1:500
7B3/transitin E7 chick retinal cells. Genbank accession no. X80877 Developmental Studies Hybridoma Bank, (Univ. Iowa). Rat monoclonal. #A2B11 1:500
FoxD3 purified in vitro translated FoxD3. Laboratory of David Raible (Univ. of Washington, Seattle). Antibody was raised in rabbit. 1:500