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Wild type FVB and LRRK2R1441G tg genotype was confirmed using PCR (A) and Sanger sequencing (B). Transgene copy number was compared to animals with known genotype to confirm hemizygous expression of LRRK2R1441G (C). Samples from each mouse were run in triplicate. Data shown are means ± s.d. n≥2 mice per group as indicated.
