Figure 7.

Functional assay of 5-azaC-treated Treg. (A) Treg and T-effectors were sorted by FACS first and stained with two different proliferation dyes (CFSE for T-effectors and VPD for Treg). 5-azaC-treated Treg were unable to inhibit the proliferation of T effectors compared with untreated Treg. 5-azaC-treated Treg were still proliferating after treatment with 5-azaC despite a reduced inhibitory function. (B) 5-azaC-treated Treg were unable to suppress cytokine secretion by T-effectors compared to untreated Treg. Interestingly co-culture of T-effectors with 5-azaC-treated Treg led to higher IL-17 secretion compared to culture with T-effector alone.