. Author manuscript; available in PMC: 2014 Aug 1.

Published in final edited form as: FEMS Microbiol Lett. 2013 Jun 26;345(1):49–55. doi: 10.1111/1574-6968.12182

Table 1.

Strains and plasmids used in this study

Strain/Plasmid	Genotype or relevant characteristic	Reference/source
E. coli K-12 strains
BW25113	Wild type; F⁻ λ⁻ Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) rph-1 Δ(rhaD-rhaB)568 hsdR514	CGSC (Keio) (Baba et al., 2006)
JW1525	BW25113 ΔmarB::Kan	CGSC (Keio) (Baba et al., 2006)
LV22	BW25113 ΔmarB	This study
plasmids
pJPBH (control)	ori colE1 lacI, Amp^r	(Barbosa & Levy, 2002)
pMB102	ori colE1 lacI lacZp::marA; Amp^r	(Pomposiello et al., 2001)
pMB102-marB	ori colE1 lacI lacZp::marB; Amp^r	This study
pMB102-marB-42-STOP	ori colE1 lacI lacZp::marB-42-stop codon; Amp^r	This study
pMB102-marB-23-STOP	ori colE1 lacI lacZp::marB-23-stop codon; Amp^r	This study
pCH58	ori pMB1, phoA fusion vector, Tet^r	(Hoffman & Wright, 1985)
pCH58-marB	ori pMB1, marB-phoA fusion, Tet^r	This study
pCH58-marB-SS	ori pMB1, marB signal sequence-phoA fusion, Tet^r	This study
pCH58-SS	ori pMB1, phoA signal sequence-phoA fusion, Tet^r	This study
pCP20	oriR101, plasmid for excision of kan and cat markers by FLP-mediated site-specific recombination; Amp^r Chl^r	(Datsenko & Wanner, 2000)