Table 2.
PhoA enzymatic activities in wild type strain BW25113 carrying plasmids containing the indicated phoA gene fusions
Plasmid | Promoter | Signal sequence | PhoA activity units‡ (SEM) |
---|---|---|---|
— | — | — | 4.3 (1.3) |
pCH58 | bla | — | 22 (6.7) |
pCH58-marB* | bla | marB | 390 (40) |
pCH58-marB-SS# | bla | marB | 410 (19) |
pCH58-SS | bla | phoA | 1600 (250) |
pCH58-marB contains full marB (except stop codon) cloned upstream of phoA.
pCH58-marB-SS contains only marB signal sequence cloned upstream of phoA.
These values are the averages of at least 6 independent experiments, with SEM in parentheses.