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. 2013 Jul 31;8(7):e70327. doi: 10.1371/journal.pone.0070327

Figure 6. Overexpression (OE) of mouse and zebrafish PrP constructs in early zebrafish embryos.

Figure 6

Embryos were microinjected with mRNAs encoding all mouse and zebrafish EGFP-tagged PrP constructs. A) OE of mouse or zebrafish WT PrPs in early embryos produces a gain-of-function phenotype characterized by asymmetric gastrulation at 6 hpf. B–D) The activities of mouse and zebrafish constructs were evaluated morphologically by quantifying the proportion of 6 hpf embryos exhibiting the OE phenotype (asymmetric gastrulation). Three independent experiments were analyzed (average n = 30 embryos). Triple asterisks [***] indicate statistically significant reduction in activity at p<0.001; one-way ANOVA test; error bars represent SEM.