Figure 2. CD45 KO macrophages have limited hyperphosphorylation of proteins in lysates compared to WT.

Lysates from day 7 cultures of BMDM were probed with anti-phosphotyrosine and anti-GAPDH as a loading control. Regions of the gel containing higher tyrosine phosphorylation in the CD45 KO lysates are indicated with an asterisk. Western blot is representative of more than three (BMDM) experiments. It is important to note that the molecular weight standards used migrate more slowly than predicted.