Figure 2. Generation of double conditional knockout mice lacking alleles of Pten and Survivin.

(A) Mouse genotype of interest was obtained by crossing mice carrying Cre transgene and floxed phosphatase region of Pten (exon V) with floxed Survivin mice. (B) Genotypes of mice (shown: 10 weeks old) were determined by PCR analysis of the tissue samples. Tail DNA extracts from cPten^-/- and Pten ^f/f mice were used as positive (+) and negative (-) controls for Cre, H₂O, water. (C) Status of Survivin deletion was also confirmed at the protein level. This is illustrated by a representative Western blot from the ventral prostate of mice from the 10-, 21-, and 37-week groups, with β-actin serving as loading control. Prostate tissue lysates from Pten ^f/f S ^f/f mice were used as normal control (ctrl).