Figure 3–

Replication of torque teno mini virus (TTMV) isolates TTMV-LY genomes after transfection produce more replicative TTMV in human embryonic kidney (HEK) 293T cells than in A549 cells as quantified by real-time qPCR. Histograms represent the mean±sd rate of replication of TTMV-LY in the cells transfected with the linearised full-length genomes. Replication of TTMV-LY was a) measured at day 3 post-transfection or b) monitored over a week following transfection. The curves in b) indicate the quantification of TTMV-LY1 in the culture medium of cells at days 0–7 post-transfection after DNaseI (Thermo Scientific, Illkirch, France) treatment and nucleic acid extraction. Results are expressed as the ratio of quantified TTMV-LY1 at day 1–7 post-transfection to quantified TTMV-LY1 at day 0. Data are shown only for TTMV-LY1, because the replication profile among the three viruses is similar.