Figure 5–

Torque teno mini virus (TTMV)-LY isolates infect the A549 alveolar cell line preferentially over the human embryonic kidney (HEK)293T cell line, as quantified by real-time PCR. a) and b) A549 cells were incubated with TTMV-LY1 for 2 h, 7 h, and 24 h with three different viral loads of 10⁵, 10⁶, and 10⁷ genome equivalents, harvested from the supernatants (SN) of transfected A549 or HEK293T cells at day 3 post-transfection. The quantity of TTMV-LY measured in culture-medium exposed cells was null. c) The quantity of replicated TTMV-LY was measured at day 0 and day 3 post-infection in the 2-h exposed A549 and HEK293T cells with a viral load of 1×10⁷ genome equivalents. Results are expressed as fold increase compared to cells exposed with the supernatants of cells exposed to the TTMV-LY genomes without transfection reagent. Data are presented as mean±sd.