Figure 5.

The effect of treatments on proliferation and mitotic index of tumors. Immunohistochemistry on paraffin-embedded tumor sections stained with antibodies for (A) proliferation marker Ki67 (magnification, x20) and (B) mitotic marker PH3 (magnification, x40). The brown areas indicate cells or areas expressing Ki67 or PH3. Tonsil was used as positive control for both Ki67 and PH3. The graphs below the images represent the comparison of proliferative and mitotic indices measured as the pixels for areas positive for Ki67 and PH3, respectively.