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. 2013 Aug;15(8):888–897. doi: 10.1593/neo.13608

Figure 6.

Figure 6

(A) A panel of glioma stem-like cultures grown as neurospheres from various GBMs was screened for the presence of TRADD by Western blot. All of the stem cell cultures express TRADD. (B) TRADD was silenced in neurosphere culture derived from GBM836 using lentiviral shRNA followed by puromycin selection, and two pools were examined. Pool 2 has better silencing of TRADD. Control shRNA (scrambled) cells were used as control. (C) While the majority of control shRNA cells are viable, silencing TRADD in glioma stemcell cultures results in cell death with increased Annexin-positive cells (P ≤ .008, two-tailed unpaired t test). (D) A second GBM primary neurosphere culture from GBM622 with silenced TRADD also shows increased Annexin-positive cells in TRADD-silenced cells compared to control shRNA cells (P ≤ .0006, two-tailed unpaired t test). (E) Western blot showing silencing of TRADD in GBM622 neurosphere cultures.