Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
GCN2 plays a key role in AAD-induced VEGF expression. (A) UM-SCC-22B cells were infected with shRNA against GCN2 (shGCN2-1 and shGCN2-2) or scrambled shRNA (scshRNA) and treated with AAD or GD for 8 hours. GCN2 and ATF4 were detected by Western blot (left panel). Percentage of GCN2 knockdown efficiency was quantified with ImageJ (right panel). (B) VEGF mRNA levels were determined using qPCR (left). VEGF secretion was quantified with ELISA (right). *P < .05.
