Figure S1.

NSun2-RNA Complex Detection and cDNA Amplification, Related to Figure 1

(A) Radiolabeled NSun2-RNA complexes run on denaturing gels and blotted onto nitrocellulose membranes. Marking indicates NSun2-RNA complexes of higher molecular weight dissected for library preparation. A range of RNaseI conditions were tested and a dilution of 1:1000 gave the best results. HEK293 cells transfected with empty vector (eV) used as a negative control to exclude contamination in the subsequent PCR step during library preparation.

(B) TBE polyacrylamide gel showing PCR miCLIP cDNA fragments after 25 cycles of amplification. DNA was stained using ethidium bromide. High- (H), Medium- (M), and Low- (L) indicates the size of excised TBE-Urea gels fragments before the amplification step.