Figure 1.

Cdc42-GTP Recruits Septins during Bud Emergence and Chasing

(A) Time-lapse analysis of Cdc42-GTP (Gic2PBD-RFP; see also Figure S1) and septin (Cdc3-GFP) dynamics in wild-type cells. Arrowhead indicates bud emergence. See also Movie S1.

(B) Average fluorescence intensity of Gic2PBD and Cdc3. Gic2PBD intensity was quantified using a threshold method (see Supplemental Experimental Procedures and Figure S2). Septin intensity was quantified using a fixed region of interest (ROI) set at the PBS. Average intensities are reported after background subtraction and normalization. Error bars represent SEM.

(C) Chasing of Cdc42-GTP cluster by septins in a latA-treated cell. See also Movie S2.

(D) Quantification of fluorescence intensity of Gic2PBD-RFP and Cdc3-GFP within the ROIs marked on (C).

Scale bars represent 3 μm.