Figure 4.

Polarized Exocytosis Could Transform a Septin Cap into a Ring

(A) De novo emergence of a tiny bud in latA-treated wild-type cells. Top: side view and 3D reconstruction showing tiny bud (Gic2PBD-RFP) and the septin ring (Cdc3-GFP). Bottom: a series of four consecutive images (2 min apart) shows the septin cap-to-ring transition (3D reconstructions).

(B) Spatial profiles of Gic2PBD and septin concentrations along the indicated in (A) line segments (blue), before and after the cap-to-ring transition.

(C) Septin ring formation in the model with exocytosis. Shown are the concentration profiles at the initiation of septin cap hollowing (left), after the septin ring formation (middle), and the 3D view (right).

See also Movie S4.