Figure 6.

Diameter of Septin Ring Strongly Correlates with the Size of Cdc42-GTP Cluster

(A) Representative images of Cdc42-GTP clusters at their peak activities before bud emergence (side-view, red) and newly formed septin rings at bud emergence (en face-view based on 3D-reconstruction, green). Cell outlines are indicated by the dotted yellow line.

(B) Septin ring diameter correlates with the Cdc42-GTP cluster size at their peak activities in WT and Cdc42 GAP mutant cells.

(C) Correlation between the septin ring diameter and Cdc42-GTP cluster size in model simulations with cytoplasmic and septin-bound GAP pools reduced to the fractions (%) indicated on the figure.

Scale bars represent 3 μm.