Table 5.

PCR and pyrosequencing primers used for DNA methylation analysis.

MOUSE GR GENE (Nr3c1) – chr18:39,649,906-39,650,025*
PCR primer – forward	GGTTTTGTAGGTTGGTTGTTATTT
PCR primer – reverse – Biotinylated	/5Biosg/TCTCTTCTCCCTAACTCCTT
Pyrosequencing primer	GGGTTTTGGAGGTAGATTTA
MOUSE BDNF PROMOTER IV (Bdnf IV) – SITES IV1-IV4 – chr2:109,532,399-109,532,715*
PCR primer – forward	TAGGATTGGAAGTGAAAATATTTATAAAGT
PCR primer – reverse – Biotinylated	/5Biosg/CCTTCAACCAAAAACTCCATTTAATCT
Pyrosequencing primer	AGAGGAGGTATTATATGATAG
MOUSE BDNF PROMOTER IX (Bdnf IX) – SITES IX5-IX1 – chr2:109,562,918-109,563,064*
PCR primer – forward	GGTGTTTGGTGTTTTAAGTAGTT
PCR primer – reverse – Biotinylated	/5Biosg/ACAAATCCTATATAACCTTTTAATTCC
Pyrosequencing primer	TGAGTAGGAGTAGTATGATAA

*Genomic coordinates are based on the UCSC Genome Browser Mouse July 2007 (NCBI37/mm9) Assembly.