Table 1.
instrument | vendor | samplea | no. files | precursor/fragment m/z toleranceb | databases used for search |
---|---|---|---|---|---|
LTQ-XL | Thermo Fisher | BSA | 280 | 1.25/0.5 | RefSeq.BOVINE |
LTQ-Orbitrap | Thermo Fisher | BSA | 53 | 10*/0.5 | RefSeq.BOVINE |
HCT Ultra | Bruker Daltonics | BSA | 24 | 1.25/0.5 | RefSeq.BOVINE |
QSTAR Elite | AB SCIEX | Bgal | 23 | 100*/0.4 | UniProt.ECOLI |
TripleTOF 5600 | AB SCIEX | Bgal | 60 | 50*/50* | UniProt.ECOLI |
LTQ-Velos | Thermo Fisher | yeast | 5 | 1.25/0.5 | SGD.orf_trans_all |
BSA (bovine serum albumin) and Bgal (Escherichia coli β-galactosidase) were purchased as protein standards. Yeast reference materials were generated as part of the NCI CPTAC program. All samples were analyzed via LC-MS/MS experiments.
Asterisk denotes mass tolerances in parts-per-million.