Fig. 3.

Characterization of TgNek1. (A) Identification of two TgNek1 splice variants by RACE PCR. Coding sequence is indicated by colored blocks, and UTRs by colored lines. The blue block in variant 2 indicates a different reading frame compared with the other two models. The asterisk marks the location of the V-A15 point mutation. (B) Plaque assays of wild-type RH, V-A15 mutant and V-A15 complemented by the pnek-TgNek1-2/sagCAT construct (V-A15 + Var.2) at indicated temperatures. (C) DNA content analysis of the complemented V-A15 clonal line (V-A15 + Var.2) at 40°C displays wild-type phenotype restoration.