Fig. 5.
Catalytic activity is required for cell-cycle-dependent localization of TgNek1-2. (A) Live imaging of transient expression of TgNek1-2-YFP driven by the α-tubulin promoter in a clonal RH line expressing Cherry-MORN1 shows a dynamic localization throughout late S phase (a), mitosis (b–d) and cytokinesis (b–e). (B) Schematic summary of TgNek1-2 localization throughout the cell cycle representing the stages shown in A. (C) Transient expression of TgNek1-2K135M-YFP driven by α-tubulin promoter in a clonal line expressing Cherry-MORN1 shows a cell-cycle-dependent localization (progressing from left to right).