Fig. 4.

Thr150 is essential for proper regulation of basal body assembly. Thr150 in the NBD was mutated to Val (Etu1p-T150V). (A) Staining for Cen1 and the KD fiber at 30°C (n = 98 and n = 86, WT and mutant respectively), and at 38°C (n = 76 and n = 87, WT and mutant, respectively). Basal body density for each strain was measured by counting Cen1 foci and is indicated in the lower right corner of each sample set (n = 25). Etu1p-T150V cells show a significant increase (P<0.01) in basal body density as compared to WT at 38°C and Thr150Val at 30°C. Percentages indicate the frequency of the observed phenotype. Scale bars: 10 µm.