Fig. 7.

Thr150 is required for proper separation and organization of basal bodies. Etu1p-T150V and WT cells were grown at 38°C for 24 hours then analyzed by transmission electron microscopy. (A) Mature basal bodies [indicated by the presence of the KD fiber (KD) and post-ciliary microtubule (PCT)] are present in abnormally close proximity. (B) Two basal bodies have assembled and inserted into the membrane abnormally close to each other. (C) Basal bodies are unusually close, and a basal body is misoriented in the cytoplasm. (D) Basal bodies have assembled unusually close to each other with no apparent organization and there is also a basal body that is disassembling from the distal end. (E) Basal bodies within a cortical row with proper spacing and organization in a wild-type cell.