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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Eur J Immunol. 2008 Sep;38(9):2464–2474. doi: 10.1002/eji.200737952

Figure 1. T-bet deficient DC fail to reprogram Th2 polarized splenocytes.

Figure 1

(A, B) T-bet deficient (T-bet KO) or wild type (WT), Th2 polarized and ovalbumin (OVA) sensitized splenocytes were co-cultured with WT bone-marrow derived DC that had been pre-incubated with OVA and either B7-DC XAb or control IgM. (A) IFN- γ and (B) IL-4 levels in the culture supernatants were analyzed by ELISA on day 3. Although B7-DC XAb induced IFN-γ and inhibited IL4 production in WT splenocytes, no such effect was seen in T-bet KO splenocytes. (C, D) Th2 polarized and OVA sensitized WT splenocytes were co-cultured with either WT or T-bet KO syngeneic bone-marrow derived DC pretreated as in (A, B). (C) IFN- γ and (D) IL-4 levels in the culture supernatants were analyzed by ELISA on day 3. T-bet KO DC failed to induce IFN-γ and downregulate IL-4 in WT splenocytes. Data displayed are pooled from three independent experiments and error bars show mean ± SEM. B7-DC XAb = XAb, Control IgM = ctrl, difference is not significant = NS. For small data points the mean is displayed as number on the graph. The # symbol indicates that the value was below the detection limit of the assay.