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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Reprod Dev. 2013 Jan 30;80(2):132–144. doi: 10.1002/mrd.22144

Figure 2.

Figure 2

COMP is a secreted protein that increases significantly during in vitro follicle growth and can be detected in culture medium. A: Scaled trends of the intensity using self-organized maps (SOMs) for the genes encoding secreted proteins during ovarian follicle maturation. Solid lines indicate the average, scaled mean intensity of the group and dotted lines represent 1 standard deviation (note that group 6 contains only Gdnf). Listed is a representative gene from each group (bottom right). See Supplemental Table 2 for the complete list of genes encoding secreted proteins. B: Gene expression profiles comparing Comp derived from the microarray analysis (black) and qPCR validation (gray). *P <0.05, **P <0.01, ***P < 0.001 relative to expression at culture day 2 (n=3 independent experiments, one-way ANOVA followed by Bonferroni's Multiple Comparison Test). C: Western blot detection of VEGFA, INHBA, and COMP in conditioned media from individual follicles cultured in vitro. Images are representative of three independent experiments.