Figure 3.
Ovarian COMP expression in vitro and in vivo. A: Image of a representative follicle cultured for 8 days and immunostained for COMP protein (i), counterstained with DAPI (ii), and photomerged (iii). Scale bar, 100 μm. Confocal microscopy image of mural granulosa cells within the follicle showing COMP localized to cytoplasmic and extracellular compartments (iv). Scale bar, 15 μm. B: COMP immunofluorescence of murine ovarian sections at different stages of the estrous cycle. Ovarian sections taken at diestrus, proestrus at 10:00 am, proestrus at 4:00 pm, estrus, and metestrus. Arrowheads signify COMP-positive staining within the ovarian surface epithelium. Scale bar, 200 μm. Images are representative of 2–3 independent experiments.