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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Neurochem. 2010 Sep 28;115(4):941–955. doi: 10.1111/j.1471-4159.2010.06984.x

Fig. 10.

Fig. 10

Effect of PHLPP1β and PHLPP1 knockdown on PKC phosphorylation. Hippocampal neurons transduced with lentivirus containing either a non-targeting shRNA (NT Virus), a shRNA virus targeting PHLPP1β (PHLPP1β KO), or shNRA’s targeting both PHLPP1α/β (PHLPP1 KO) and treated with 100 ng/mL IGF-1 for 20 min. (a and b) Representative western blots (n = 3) show the effect of knockdown on PKC phosphorylation. (c and d) Densitometry of western blots (n = 6) show PHLPP1β knockdown significantly reduces PKC total levels and phosphorylation at Ser657. (e and f) Densitometry of western blots (n = 6) show PHLPP1 knockdown does not alter PKC total levels or phosphorylation. Data are significant at ***p < 0.0001.