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. 2013 Jan 10;5(1):15–26. doi: 10.3390/cancers5010015

Figure 3.

Figure 3

A deficient HIF-1α expression increases chemosensitivity to 5-FU in the gastric cancer cells MKN-45. (A) A Western blot analysis of HIF-1α in the HIF-1α-deficient gastric cancer cell line MKN45-KD in which HIF-1α siRNA plasmids were transfected. Hypoxic induction of HIF-1α was completely knocked down in MKN45-KD compared with that observed in the control cell line MKN45-SC. (B) Effects of HIF-1α knockdown on sensitivity in xenografts of nude mice. 5-FU treatment was administered three times a week starting on Day 17. The tumor size before treatment was significantly larger in the MKN45-KD tumors than in the MKN45-SC tumors. The response rate to 5-FU, which was estimated based on tumor size on Day 35, was significantly better in the KD tumors than in the SC tumors (41.7% ± 38.3% vs. 141.38 ± 48.1%). (C) A TUNEL assay showed a significant increase in the number of apoptotic cells with nuclear staining in the HIF-1α KD tumors after 5-FU treatment.