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. 2013 Apr 3;5(2):334–356. doi: 10.3390/cancers5020334

Figure 1.

Figure 1

RAB25 is a ZEB1 target gene. (A) RAB25 expression positively correlated with E-cadherin but negatively with ZEB1 in a series of lung cancer cell lines. RNA expression was measured by quantitative real-time PCR in 22 NSCLC cell lines, two NHBE cultures, and two immortalized human airway primary cell lines (BAES2B and FC6625-2 3KT). Cells are ranked from left to right with increasing RAB25 mRNA level. Values are expressed as percent of the geometric mean between GAPDH and actin mRNA. The experiment was done twice with qRT-PCR in duplicate. (B) RAB25 mRNA level is decreased by ZEB1 overexpression in H358 FlipIn ZEB1 cells (left) and by TGFβ treatment in H358 EV control cells (right). Values are expressed as percent of GAPDH for three independent experiments with qRT-PCR in duplicate. Bars = SD. (C) Western blot: RAB25 protein level is decreased by ZEB1 overexpression during 1 to 5 days of DOX treatment in H358 FlipIn ZEB1cells. E-cadherin is decreased as well. Actin is the loading control. Protein molecular weights are indicated in kDa on the left.