Table 2.
Antibodies for ChIP, western blot, immunocytochemistry, and immunohistochemistry. References, quantities and working dilutions are indicated. IF: immunofluorescence, WB: western blot, TMA: tissue microarray.
| Primary antibodies | Reference | Lot | ChIP | IF | WB | TMA |
|---|---|---|---|---|---|---|
| Rabbit control serum | Sigma I5006 | 10 µg | ||||
| Rabbit anti-ZEB1 | Santa Cruz Biotechnology, H102 | D2010 | 10 µg | 1:50 | 1:50 | |
| Mouse monoclonal anti-ZEB1 | R&D MAB 6708 | CEZB0111021 | 1:50 | |||
| Rabbit anti-acetyl histone H3K9 | Active Motive 39137 | 01008001 | 10 µL | |||
| Rabbit anti-acetyl histone H3K27 | Active Motive 39133 | 3161003 | 10 µg | 1:500 | 1:20,000 | 1:50 |
| Rabbit anti-acetyl histone H3 | Millipore 06-599 | DAM1823380 | 10 µg | 1:500 | 1:20,000 | 1:50 |
| Rabbit anti-acetyl histone H4 | Millipore 06-866 | JBC1873473 | 10 µg | 1:20,000 | ||
| Rabbit monoclonal anti-E-cadherin | Cell Signaling 24E10 | 2 | 1:100 | 1:1,000 | ||
| Mouse anti-E-cadherin | B&D 610181 | 85521 | 1:100 | |||
| Anti-Rab25 | Sigma R8532 | 1:1,000 | ||||
| Anti-actin | Sigma | 1:50,000 |