Table 4.

The results of topoisomerase IIα immunohistochemistry are showing a frequent link between a diffuse staining and an allelic imbalance at TOP2A locus (six patients). Among these six patients, four were presenting a TOP2A amplification (QCR analyses).

Patients	Topoisomerase IIα iummunohistochemistry				Allelotyping	QPCR
	Positive cells (%)	Staining intensity	Localization	Assessment
Patient 1	10%	strong	nucleus	diffuse	homozygous	normal
Patient 25 *	50% (focal)	strong	nucleus	heterogeneous	normal	normal
Patient 26	10% (focal)	moderate	nucleus	heterogeneous	normal	normal
Patient 28	40–50%	strong	nucleus	diffuse	allelic imbalance	deletion
Patient 29	40% (focal)	strong	nucleus	heterogeneous	normal	normal
Patient 30	40% (focal)	strong	nucleus	heterogeneous	normal	normal
Patient 31	20%	strong	nucleus	diffuse	allelic imbalance	amplification
Patient 32	40% (focal)	strong	nucleus	heterogeneous	normal	normal
Patient 38	20%	strong	nucleus	diffuse	allelic imbalance	amplification
Patient 44	50% (focal)	moderate	nucleus	heterogeneous	normal	normal
Patient 47	30% (focal)	strong	nucleus	heterogeneous	normal	normal
Patient 55	50% (focal)	strong	nucleus	heterogeneous	homozygous	normal
Patient 64	50%	moderate	nucleus	diffuse	allelic imbalance	amplification
Patient 65	70%	strong	nucleus	diffuse	normal	normal
Patient 66*	30%	strong	nucleus	diffuse	allelic imbalance	amplification
Patient 67	60%	strong	nucleus	diffuse	allelic imbalance	amplification
Patient 68	50%	strong	nucleus	heterogeneous	allelic imbalance	deletion

The patient marked with * are those shown in Figure 4 for positive topoisomerase IIα immunohistochemical staining.