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. 2013 Jun 4;5(2):662–675. doi: 10.3390/cancers5020662

Table 5.

Primers for the semi-quantitative PCR of TOP1, TOP2A and TOP2B genes.

Genes Forward primers Reverse primers
Target genes
TOP1 5'-ATGGGTACAGTGTGCT-3' (intron 19) 5-'AGTTTGGAGGTTCCCAG-3' (exon 20)
TOP2A 5'-GCCATTGGCTGTGGTATTG-3' (exon 11) 5'-GAGAAGCTTCTCGAACATTGAG-3' (exon 12)
TOP2B 5'-GATTGGGTACTAGTACAGCT-3' (exon 16) 5'-GAATAGAAGGTAGGGGGATG-3' (intron 16)
Reference genes
APP 5'-TCAGGTTGACGCCGCTGT-3' 5'-ACCCCAGAGGAGCGCCACCTG-3'
DCK 5'-GCCGCCACAAGACTAAGGAAT-3' 5'-AGCTGCCCGTCTTTCTCAGCCAGC-3'