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. 2013 Jun 18;5(7):1298–1308. doi: 10.1093/gbe/evt094

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© The Author(s) 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 2.— — PCR verification of mt minichromosomes of Haematopinus suis and H. apri. (A) Lanes 1 and 14: low mass ladder (LML). Lanes 2 and 13: molecular weight marker VII (MWM). Lanes 3–10: amplicons from eight minichromosomes of H. suis (B2311), rrnS-trnC, trnL₁-rrnL, nad2-trnI-cox1-trnL₂, trnR-nad4L-nad6-trnM, trnK-nad4-atp8-atp6-trnN, trnE-cob-trnV, trnQ-nad1-trnT-trnG-nad3-trnW, and trnD-trnY-cox2-trnS1-trnS2-trnP-cox3-trnA. Lanes 11–12: PCR amplicons from two minichromosomes of H. apri (B2418), rrnS-trnC, and trnQ-nad1-trnT-trnG-nad3-trnW. Genes where PCR primers were designed are in bold. (B) Lane 1: amplicon from trnH-nad5-trnF minichromosome of H. suis (B2311). Lanes 2, 9, and 15: 1-kb ladder. Lanes 3–8 and 10–14: PCR amplicons from seven minichromosomes of H. apri (B2418), trnK-nad4-atp8-atp6-trnN, trnK-nad4-atp8-atp6-trnN, nad2-trnI-cox1-trnL₂, nad2-trnI-cox1-trnL₂, trnD-trnY-cox2-trnS1-trnS2-trnP-cox3-trnA, trnD-trnY-cox2-trnS1-trnS2-trnP-cox3-trnA, trnE-cob-trnV, trnH-nad5-trnF, trnR-nad4L-nad6-trnM, trnR-nad4L-nad6-trnM, and trnL₁-rrnL. (C) Amplicons by pig-lice-specific primers, PGC1F-PGC1R and PG12SF-PG12SR, from the entire nad2-trnI-cox1-trnL₂ minichromosome (4.5 kb, Lane 1) and the entire rrnS-trnC minichromosome (3.5 kb, Lane 4) of H. suis. Lane 2: LML. Lane 3: 1-kb Ladder. (D) Amplicons from the coding regions of mt minichromosomes of H. suis and H. apri. Lanes 1 and 11: LML. Lanes 2 and 10: MWM. Lanes 3–4: H. suis from Australia (B2311). Lanes 5–6: H. suis from Poland (B2419). Lanes 7–8: H. apri from Japan (B4218). Lane 9: H. suis from China (B2572).