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. 2013 Mar 21;22(16):2326–2340. doi: 10.1089/scd.2012.0489

FIG. 3.

FIG. 3.

(A) Western blot analysis of non-fused insulin-like growth factor (IGF) expression using an anti-tetra-His mAb for detection. M–Magic marker; 1–IGF-1-HK (clone 4); 2–IGF-1-HK (clone 6) (B) Western blot analysis of non-fused IGF-1 expression using an anti-IGF-1 mAb for detection and run under non-reducing conditions. M–Magic marker; 1–IGF-1-K–soluble protein. (C) Western blot analysis of IGF-1-LicKM fusion products expression using an anti-tetra-His antibody for detection. M–Magic marker; 1–10 ng HAI standard [hemagglutinin from A/Indonesia/05/05 (H5N1) strain of influenza virus]; 2–25 ng HAI standard; 3–50 ng HAI standard; 4–IGF-1-2E–soluble protein; 5–IGF-1-2E–total protein; 6–IGF-1-3E–soluble protein; 7–IGF-1-3E–total protein.