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Western blot analysis of non-fused stem cell factor (SCF) product expression. (A) Detection with an anti-tetra-His mAb. M–Magic marker; 1–SCF-HK (clone 1); 2–SCF-HK (clone 2) (B) Detection with an anti-SCF mAb. M–Magic marker; 1–SCF-K–soluble protein; 2–SCF-K–total protein. (C) PNGase F treatment of purified SCF-HK, detected with an anti-SCF mAb. M–Magic marker; 1–non-treated SCF-HK; 2–PNGase F-treated SCF-HK.
