Table 1.
Assessment of Biological Activity of Plant-Produced Cytokines In Vitro in Cell Proliferation Assays
| R&D Systems | EC50 (ng/mL) | NRMSE | FhCMB | EC50 (ng/mL) | NRMSE |
|---|---|---|---|---|---|
| SCF | 6.1 | 5% | SCF-HK | 6.4 | 22% |
| EPO | 0.3 | 11% | EPO-2E | 0.4 | 10% |
| IL-3 | 0.1 | 6% | IL-3-2E | 1.2 | 5% |
| IGF-1 | 0.2 | 14% | IGF-1-2E | 1.7 | 12% |
Dose-dependent cytokine effect on cell proliferation was assessed by increasing concentrations of EPO-2E, SCF-HK, or IL-3-2E added to TF1 cells, or increasing concentrations of IGF-1-2E added to MCF-7 cells. EC50 values for the plant-produced cytokines calculated from the data obtained in the linear dose-response range were compared with the EC50 of corresponding commercial standards.
NRMSE, normalized root mean squared error; EPO, erythropoietin; SCF, stem cell factor; IL-3, interleukin 3; IGF-1, insulin-like growth factor-1.