Figure 2.

EB differentiation deviated toward ectoderm. (a) Experimental protocol used for differentiation. After EB formation for 4 days, Math1-ES-derived EBs were cultured with or without Dox for 2 weeks. (b) Photographs of EB outgrowths after 2 weeks of cultivation. Arrows indicate beating cells. Bar=100 μm. (c) Numbers of beating focuses with and without 2 weeks of exposure to Dox. (d) Analysis of expressions of non-differentiation markers (Oct-3/4, UTF-1), ectodermal markers (nestin, NF-L), mesodermal markers (ζ-globin, Nkx2.5), and endodermal markers (AFP, TTR) in EB outgrowths after cultivation with or without Dox for 2 weeks. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; N.D., not detected