Figure 4.

Immunocytochemical analysis of HC-related markers in the context of Math1 expression. (a) Math1-ES-derived EB outgrowths were treated with Dox for 2 weeks, then examined for expressions of myosin6, Brn3c, and α9AchR in the context of Math1 expression. Cells intensively positive for Math1 were easily distinguished from other vaguely positive cells in visual observations (O) and also selectively evident in modified observations (M). Bar=50 μm. (b) Using an immunocytochemical method, the expressions of My6, Brn3c, and α9AchR were investigated among cells intensively positive for Math1 in Math1-ES-derived EB outgrowths treated with Dox for 2 weeks. The three markers were simultaneously expressed at high frequencies