Figure 5.

Mechanotransduction channels and stereocilia-like structures in HC-like cells. (A) Phalloidin-positive cells were found in Math1-ES-derived EB outgrowths treated with Dox. Bar=40 μm. (B) Math1-ES-derived EB outgrowths cultured with Dox were treated with FM1-43FX dye and stained with Brn3c after fixation. A marked number of FM1-43FX-positive cells were identified in the EB outgrowths. Insets: high magnification views. Bar=50 μm. (C) Math1-ES-derived EB outgrowths cultured with and without Dox for 2 weeks were examined using a SEM. (c and d) Cells with distinct stereocilia-like structures were observed in the EB outgrowths cultured with Dox, while (a and b) no cells presenting such structures were found among those cultured without Dox. (b and d): High magnification views of the areas in panels (a and c), respectively (red arrows). Bars=20 μm (a and c) and 2 μm (b and d)