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. 2013 Jul 4;4(7):e700. doi: 10.1038/cddis.2013.230

Figure 6.

Figure 6

Roles of Math1 in induction of HC-like cells from ES cells. (a, b) The effects of Math1-specific siRNA on differentiation of HC-like cells from ES cells using the HIST2 method were examined with quantitative PCR. Suppression of Math1 by Math1-specific siRNA inhibited the expression of Brn3c, an HC-related marker (a), and Brn3c-immunopositive cells were also decreased (b). *P<0.05 (c) The promoter activity of Brn3c was examined following transfection of a reporter Brn3c-promoter plasmid. Undifferentiated Math1-ES cells (Undiff. ES) and Math1-ES-derived EB outgrowths cultured with Dox for 14 days (EB outgrowths) were transfected with the pEZX-PG04-Brn3c reporter plasmid and further cultured with or without Dox. After 48 h, cell-free conditioned medium samples were assayed for Gluc and SEAP using a Secrete-Pair Dual Luminescence Assay Kit. The promoter activity of Brn3c was positively regulated in the cultures containing Dox in both undifferentiated ES cells and EB outgrowths. *P<0.05. DAPI, 4,6-diamidino-2-phenylindole