Figure 3.

(a) Western-blot analysis of CRFR expression. CRFR1 and CRFR2 protein levels were determined using rabbit anti-CRFR1 and rabbit anti-CRFR2 monoclonal antibodies with a mouse anti-α-tubulin monoclonal antibody used as a loading control. Bands with sizes corresponding to 50 kDa (CRFR1), 47 kDa (CRFR2) and 55 kDa (α-tubulin) were detected. (b) Observed bands were quantified by densitometry and CRFR expression normalized to that of α-tubulin. Data are presented as mean±S.E.M. of three independent experiments