Table 1. Sequences of primers used for PCR amplification of cDNA and predicted amplicon sizes.
Gene | Primer sequence (5′→3′) | Expected product size (bp) | PCR protocol | Cycles |
---|---|---|---|---|
UCN | CAGGCGAGCGGCCGCG | 146 | 94°C/57°C/72°C | 33 |
(Human) | CTTGCCCACCGAGTCGAAT | |||
GAPDH | CCTGCTTCACCACCTTCTTG | 437 | 94°C/58°C/72°C | 30 |
(Human) | CATCATCTCTGCCCCCTCTG | |||
CRH-R1 | ACAAACAATGGCTACCGGGA | 280 (R1α) | 94°C/58°C/72°C | 45 |
(Human) | GGACCACGAACCAGGTGGCG | 367 (R1β) | ||
CRH-R2 | AGCCCATTTTGGATGACAAG | 180 | 94°C/58°C/72°C | 45 |
(Human) | AGGTGGTGATGAGGTTCCAG | |||
CRH-R2α | TCCACAGCCTGCTGGAGGCC | 239 | 94°C/58°C/72°C | 45 |
(Human) | CTCCAAGCATTCTCGATAGGC | |||
CRH-R2β | CCTCCTCTACGTCCCACACC | 310 | 94°C/58°C/72°C | 45 |
(Human) | CTCCAAGCATTCTCGATAGGC | |||
CRH-R2γ | TGGGAAGAGAGCCTTGGCC | 212 | 94°C/58°C/72°C | 45 |
(Human) | CTCCAAGCATTCTCGATAGGC |