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. 2013 Jul 11;4(7):e717. doi: 10.1038/cddis.2013.231

Table 1. Sequences of primers used for PCR amplification of cDNA and predicted amplicon sizes.

Gene Primer sequence (5′→3′) Expected product size (bp) PCR protocol Cycles
UCN CAGGCGAGCGGCCGCG 146 94°C/57°C/72°C 33
(Human) CTTGCCCACCGAGTCGAAT      
GAPDH CCTGCTTCACCACCTTCTTG 437 94°C/58°C/72°C 30
(Human) CATCATCTCTGCCCCCTCTG      
CRH-R1 ACAAACAATGGCTACCGGGA 280 (R1α) 94°C/58°C/72°C 45
(Human) GGACCACGAACCAGGTGGCG 367 (R1β)    
CRH-R2 AGCCCATTTTGGATGACAAG 180 94°C/58°C/72°C 45
(Human) AGGTGGTGATGAGGTTCCAG      
CRH-R2α TCCACAGCCTGCTGGAGGCC 239 94°C/58°C/72°C 45
(Human) CTCCAAGCATTCTCGATAGGC      
CRH-R2β CCTCCTCTACGTCCCACACC 310 94°C/58°C/72°C 45
(Human) CTCCAAGCATTCTCGATAGGC      
CRH-R2γ TGGGAAGAGAGCCTTGGCC 212 94°C/58°C/72°C 45
(Human) CTCCAAGCATTCTCGATAGGC