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AR activates DAX-1 gene promoter. (a) MCF-7 cells, transfected with DAX-1-Luc-plasmid, were treated with 10−8 M Mb and/or 10-6M OH-Fl, (24 h). (b) HEK293 cells, co-transfected with DAX-1-Luc-plasmid plus increasing amounts of AR or ARCys-574→Arg plasmids as indicated, were treated with 10−8 M Mb for 24 h. Immunoblotting for AR and β-actin (loading control) expression. *P<0.01; n.s., nonsignificant
