Figure 4.

Representative immunoblot blot analysis of the TPO/MPL/JAK2 signaling pathway in MKs treated or not with abexinostat. CD41⁺ cells were sorted at day 7 of culture, seeded in serum-free medium with TPO with or without 50 nM abexinostat for 4 additional days. Then, MKs were TPO-deprived 12 h in the presence or absence of 50 nM abexinostat and then stimulated for indicated times with or without 50 nM abexinostat. Phosphorylation of STAT5, STAT3 and ERK was evaluated by western blot as well as the JAK2 level. Actin was used as a loading control