Fig. 3.

Cybb^−/− Nos2^−/− mice exhibit serum antibodies directed against their commensal flora. Serum and feces were collected from 8-week-old Cybb^−/− Nos2^−/− mice and co-housed C57BL/6 controls. Isolates of E. faecalis, Staphylococcus saprophyticus and Stentrophomonas maltophilia were obtained by aerobic culture from feces and pure cultures were stained with serum from Cybb^−/− Nos2^−/− and control C57BL/6 mice. Anti-bacterial IgG1 was quantified by flow cytometry. n=5 C57BL/6 controls and 5 CybbNOS2 mice. Representative data from two independent experiments are shown.