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Specific activity of protease inhibitor extracted with different extractants.
| Serial No. | Extraction medium | Specific protease inhibitor activity (units/mg protein) |
|---|---|---|
| 1 | Sodium chloride (15%) | 12.20 ± 0.05 |
| 2 | Hydrochloric acid (0.05 M) | 16.60 ± 0.04 |
| 3 | Sodium hydroxide (0.2%) | 11.60 ± 0.04 |
| 4 | Distilled water | 56.60 ± 0.23 |
| 5 | Phosphate buffer (0.1 M) | 30.20 ± 0.10 |
