Figure 2.

Gene–environment interaction during dsRNA-induced behavioural fever. (a) Differential expression levels of 156 (fold change greater than 2) dsRNA-induced transcripts common to both constant conditions (Tⁱ) or in a thermal gradient (T^d). Q₁₀ values were calculated and shown in four groupings relative to intensity. Solid curves, red (T^d) or blue (Tⁱ) denote specific mRNA abundances relative to environmental condition. (b) qRT-PCR quantification of specific anti-viral mRNA accumulation over a 72 h time period post-dsRNA challenge. Values shown are maximal mRNA relative abundance ratio (T^d/Tⁱ) in dsRNA challenged fish (n = 6) mean ± s.d. Two-way ANOVA: (i) Stat-1a, (ii) Stat-1b, (iii) Irf7, (iv) Gig2, (v) Trim25 and (vi) Somatostatin (interaction values). Letters represent comparisons (a,b,c) and significance is Bonferroni post-hoc test (*p < 0.05; **p < 0.01; ***p < 0.001).