Figure 1.

HA and CS form part of the cell surface layer of ciGEnCs. ciGEnCs grown on coverslips were left untreated (A–C, E–G) or were treated with 250 μg/mL hyaluronidase (D) or 0.1 mU/mL chondroitinase (H). The cells were then fixed and immunostained with biotinylated HABP (A, C, and D) (green) or no HABP (B) in conjunction with streptavidin–Alexa Fluor 488 and anti-CS (green) (B, G, and F) or matched IgM control (F) in conjunction with anti-mouse IgM Alexa Fluor 488 and counterstained with DAPI (blue). Three-dimensional images were reconstructed from confocal Z-stacks (A, B, E, and F) or images were acquired with an upright fluorescence microscope (C, D, G, and H).