Figure 2.

Specificity of hyaluronidase and chondroitinase. ciGEnCs, grown on 96-well plates, were treated with chondroitin ABC lyase for 2 hours at 37°C (A and B) or with hyaluronidase for 1 hour at 37°C (C and D) and then were fixed and immunostained with either biotinylated HABP (B and C) or anti-CS (A and D). The last concentration at which enzyme significantly affected fluorescence intensity is indicated by an arrow. Data were analysed using one way ANOVAs [overall P < 0.0001 (A), P < 0.001 (B), P < 0.0001 (C), P < 0.01 (D)]. Data are expressed as means ± SEM. n = 6. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001, Dunnett’s multiple comparison post hoc test.