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. 2013 Aug;183(2):604–616. doi: 10.1016/j.ajpath.2013.04.019

Figure 5.

Figure 5

The functional contribution of CS to ciGEnC barrier properties. A: ciGEnCs were grown on tissue culture inserts or on ECIS arrays. Baseline electrical resistance was measured before treatment with chondroitinase at 37°C or with CS. On inserts, the electrical resistance was normalized to baseline conditions. n = 5. B: The passage of FITC-labeled BSA across the monolayer was then monitored for three consecutive hours from 2 hours of treatment. The fluorescence intensity of the aliquots was quantified, and data were expressed as cumulative FITC-BSA over time. n = 4. C: Monolayer integrity of ciGEnCs at the various doses of chondroitinase was assessed by VE-cadherin immunostaining. n = 4. D: On the ECIS arrays, CS or vehicle was added directly to the monolayer, and resistance was measured in real time over 24 hours. n = 4. A analyzed by one way ANOVA (overall P < 0.001), B analysed by repeated measures ANOVA (overall P < 0.05) and C by unpaired t-test (overall P < 0.001). Data are expressed as means ± SEM. Bonfferoni post hoc tests indicated on graphs. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.